Effects of Picloram,ABA and TDZ on Somatic Embryogenesis of Banana

Establishment of a stable embryogenic cell suspension(ECS)is a prerequisite for biotechnological breeding of banana.Although many important progress has been made,but application of embryogenic cell suspension for genetic improvement of Musa is limited because of low induction percentage of embryogenoic callus,low conversion frequence of plant regeneration from the somatic embryos.Therefore,an optimal protocol for embryogenic cell suspensions is still required to establish.In this study the effects of Picloram,ABA and TDZ on somatic embryogenesis of banana from young male flower of Musa acuminata cv.Mas(AA)were investigated.Results showed that the induction percentage of embryogenic calli reached 15.6% when 2,4-D in the callus induction medium was substituted by 8.28 μmol/L picloram.However,the addition of ABA into somatic embryo development medium inhibited somatic embryogenesis from embryogenic cell suspension and resulted in callus formation from somatic embryos.Treatment with TDZ at concentration of 0.2 μmol/L resulted in increase of the percentage of germination of somatic embryos from 17.28% to 72.77% and the plant conversion ratio from 16.49% to 67.05%,respectively.