The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV

In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection,120 clinical samples were detected by the two methods.According to the study released by the NCBI swine fever virus strain Shi-men E2 gene sequence,design and synthesis of a pair of detection of classical swine fever virus E2 gene PCR primer.Submitted to the 120 suspected swine fever disease compound extracted total RNA,after one-step RT-PCR amplification of E2 gene,checked out 32 samples were expected to contain swine fever antigen,positive rate was 26.7%.ELISA antigen detection kit checked out 38 samples were expected to contain swine fever antigen,positive rate was 31.7%.One-step RT-PCR detected 32 positive samples were also positive in the detection of ELISA.The establishment of classical swine fever virus RT-PCR detection of nucleic acid and antigen detection ELISA.Pathogen detection of classical swine fever supplied technical support for the provision of Yunnan.