The Cholinergic-inducing Effect of BMP4 on Rat′s Cerebral Neural Stem Cells

The cholinergic-inducing effect of BMP4 on isolated and cultivated rat′s cerebral neural stem cells(NSC) was examined. NSC isolated from two months old rat′s brain region like hippocampus and striatum was cultivated in a DMEM/F12 medium containing EGF and bFGF, and was identified with morphological character and nestin immunocytochemistry test.After 24 hours, cultivating the NSC with the BMP4-added medium for 7-8 days, then the microscopical change were observated, ChAT and nestin double-labelling immunocytochemistry test was done. Results showed that about 34% NSC of neuron-like character was observed by microscope in the paper.That ChAT-positive cells coexist with nestin-positive cells was found by immunocytochemistry test.There were 28% ChAT-positive cells and 38% nestin-positive cells in the study.Cholinergic neurons differentiated from NSC could be induced by adding BMP4 to the medium.