Identification and Analysis of Brucella 80/23 Strain Isolated in Xinjiang with MLVA-16 Typing

To analyze genotype of Brucella 80/23 strain isolated in Xinjiang,multilocus variable-number tandem-repeat analysis(MLVA-16) and conventional biological identification method were used in this study.Comparative analysis was conducted between our results and Brucella 530 from database(http://mlva.upsud.fr/MLVA),and further phylogenetic tree was constructed by using UPGMA.The results showed that the Brucella 80/23 strain was identified as B.melitensis serotype 3 strains by the conventional identity method,and MLVA method displayed Brucella 80/23 strain was close to Bruce0261,belonging to Eastern Mediterranean type,B.melitensis serotype 1 strains.There is the same species between two identity methods.The conclusion was that,compared with the conventional biological identification method,MLVA-16 has higher accuracy for the type and strain of Brucella biological differences.MLVA-16 can provide the basis for traceability and evolutionary relationship of the brucellosis epidemic strain.