ScholarLens Back to search
2012•Unpublished venueRequires access

Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node

Hao RongXua, Shao Qun, Yang SuXin, Jicun Li, Xianzhong Feng, Zhao Yanxiu

Open publisher page 0 citations

Abstract

To optimize the system of Agrobacterium-mediated soybean[Glycine max(L.) Merrill]transformation,effects of 13 cultivars and 2 selection agents were studied during the process of shoot regeneration and transformation.The results showed that the cultivar 'Shanning 14' induced the highest number of multiple shoot among tested 13 cultivars,and hygromycin B was better than glufosinate during transformant selection of cultivar 'Shanning 14'.The optimal transformant selection scheme was a gradient concentration of hygromycin B from 3 to 8 mg·L-1.The integrations of UidA(GUS)gene into the transformant genome were demonstrated by histological staining,PCR application and sequencing analysis.Transformation efficiency was 3.2% based on the number of transformed events.

About this research paper

What this paper is about

To optimize the system of Agrobacterium-mediated soybean[Glycine max(L.) Merrill]transformation,effects of 13 cultivars and 2 selection agents were studied during the process of shoot regeneration and transformation.The results showed that the cultivar 'Shanning 14' induced the highest number of multiple shoot among tested 13 cultivars,and hygromycin B was better than glufosinate during transformant selection of cultivar 'Shanning 14'.The optimal transformant selection scheme was a gradient concentration of hygromycin B from 3 to 8 mg·L-1.The integrations of UidA(GUS)gene into the transformant genome were demonstrated by histological staining,PCR application and sequencing analysis.Transformation efficiency was 3.2% based on the number of transformed events.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

To optimize the system of Agrobacterium-mediated soybean[Glycine max(L.) Merrill]transformation,effects of 13 cultivars and 2 selection agents were studied during the process of shoot regeneration and transformation.The results showed that the cultivar 'Shanning 14' induced the highest number of multiple shoot among tested 13 cultivars,and hygromycin B was better than glufosinate during transformant selection of cultivar 'Shanning 14'.The optimal transformant selection scheme was a gradient concentration of hygromycin B from 3 to 8 mg·L-1.The integrations of UidA(GUS)gene into the transformant genome were demonstrated by histological staining,PCR application and sequencing analysis.Transformation efficiency was 3.2% based on the number of transformed events.

Key concepts: Transformation (genetics), Agrobacterium, Cultivar, Biology, Hygromycin B, Shoot, Glufosinate, Agrobacterium tumefaciens

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node — Research Paper | ScholarLens