Effects of constant magnetic field on proliferation and apoptosis of human umbilical vein endothelial cells intervened by hydrogen peroxide

Objective To investigate effects of constant magnetic field (CMF) on proliferation and apoptosis of human umbilical vein endothelial cells (HUVEC) intervened by hydrogen peroxide (H 2O 2) Methods The third generation of cultured HUVEC were used The cells were divided into six groups: control group, 100 μmol/L H 2O 2group, H 2O 2with one, five, ten or 50 Gs of CMF groups Samples were collected 48 hours later Cell proliferation was measured by MTT chromatometry and apoptosis was determined by propidinm iodide staining on flow cytometry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) Results Hydrogen peroxide of 100 μmol/L inhibited proliferation and induced apoptosis of HUVEC In one, five and ten gauss group, proliferation of cells was significantly higher than that in H 2O 2group ( P 0 05), whereas apoptosis of cells was significantly lower than that in H 2O 2 group ( P 0 05) There was no difference in cell proliferation and apoptosis between 50 Gs group and H 2O 2group ( P 0 05) Conclusions Hydrogen peroxide of 100 μmol/L can inhibit proliferation and induce apoptosis of HUVEC One gauss to ten gauss of CMF can intensity dependently antagonize the effects of hydrogen peroxide on HUVEC, increase the proliferation and decrease apoptosis of HUVEC