Expression of Th cytokines in peripheral blood before and after splenectomy in immune thrombocytopenia patients

AIM: To investigate the changes of Th cytokines before and after splenectomy in immune thrombocytopenia(ITP) patients.METHODS: The QuantiGene Plex method was used to measure the mRNA expression of Th1[interleukin-2(IL-2) and interferon-γ(IFN-γ)],Th2(IL-4,IL-5,IL-6 and IL-10),Th3(transforming growth factor β1,TGF-β1),and Th17(IL-17) cytokines in peripheral blood of ITP patients before and after laparoscopic splenectomy and those in peripheral blood of healthy controls.RESULTS: The mRNA level of IL-2 was significantly decreased in ITP patients before operation compared with the healthy controls,whereas IL-17 was obviously over-expressed.No significant difference of the other cytokines between preoperative group and the normal controls was found.After splenectomy,the expression levels of both IL-2 and TGF-β1 were significantly higher than those in preoperative group and the normal controls.IL-2 was also significantly increased after operation,but was still lower than that in the normal controls.No significant difference of other cytokines between postoperative group and healthy controls was observed.In addition,The Th1 cytokines(IL-2 and IFN-γ) were found to be positively correlated(r=0.647,P0.01) in preoperative patients,while no correlation was found between the other cytokines.There was a positive correlation between IL-2 and IFN-γ(r=0.787,P0.01) in postoperative patients.IL-17 also had positive correlations with IL-2(r=0.554,P0.01) and IFN-γ(r=0.461,P0.05) in ITP patients after operation,respectively.CONCLUSION: There is an imbalance of Th cytokines in ITP patients.The mechanism of splenectomy for treating ITP may be associated with the balance regulation of Th cytokines.