Virulence,pathogenicity and spread mode of a isolated goose paramyxovirus(GPMV)

A goose paramyxovirus(GPMV) was isolated from a gang of died geese due to disease by the methods of serology and biology.The serological identification indicated that the isolated virus was a avian paramyxovirus I(PMV-I).The virulence tests of MDT,ICPI,and IVPI showed that the isolated GPMV was a strong virus and its strength was similar to F48E9 of chicken.Sequence analysis showed that the predicted amino acid sequence of the fusion protein cleavage site was a typical virulent type,112K-R-Q-K-R-F117.Some chickens,hens,and geese with different level of the newcastle disease virus(NDV) antibody were infected with the isolated GPMV.The results showed that the isolated GPMV was similarity to the infection of the virulent NDV strains.It not only caused the typical ND,but also caused the non-typical ND.The morbidity,the mortality,the clinical signs,and the pathological changes were related to the immune status of chickens and geese.The infection test of different ways showed that the isolated GPMV infected chickens by some channels such as digestive tract,breath tract,straight contact,etc.