The research of glucose and lipid metabolism in lipoprotein lipase gene knockout heterozygous mice

Objective To study the role of lipotoxicity in the development of type 2 diabetes through lipoprotein lipase gene knockout heterozygous mice.Methods Three groups were divided,including wild type c57 mice(16 weeks),LPL gene knockout heterozygous mice(LPL~(+/-) mice)(16 weeks) and LPL~(+/-) mice(28 weeks).We compared the level of blood lipid among the three groups,and then undertook intraperitoneal glucose tolerance test(IPGTT) to study the state of insulin resistance and the function ofβcell.Finally,the levels of lipid of liver,skeletal muscle and pancreas were compared. Results There was significant difference in the weight of visceral adipose tissue between the control and LPL~(+/-) mice at age of 16 weeks(P0.05).The level of TG in liver,the weight and percentage of subcutaneous adipose tissue and visceral adipose tissue in 28-week-old LPL~(+/-) mice was significantly higher than in 16-week-old LPL~(+/-) mice(P0.05).The level of fasting plasma glucose(FBG),area under the curve of serum(AUC),fasting insulin(FINS),HOMA-β,HOMA-IR were higher in 16-week-old LPL~(+/-) mice than in the control,but these changes had no statistical significance.28 weeks old LPL~(+/-) mice had higher level of FBG,AUC,Fins,HOMA-β,HOMA-IR than did 16 weeks old LPL~(+/-) mice.The change of the level of FIns and HOMA-IR was statistically significant(P0.05).The LPL~(+/-) mice at age of 28 weeks had higher level of AUC,FINS,HOMA-IR than the control(P0.05). Conclusion The lipid metabolism abnormalities in LPL~(+/-) mice are aggravated along with aging,and then their glucose metabolism deteriorates.