Effect of application of different bio-organic fertilizer with tobacco specific chemical fertilizer on the amount of microbe and enzyme activities in the rhizosphere soil of flue-cured tobacco

Field experiments were carried using tobacco-specific chemical fertilizer as control,to study the impact of application of four different bio-organic fertilizer with tobacco specific chemical fertilizer in rhizosphere soil microorganisms and enzyme activities of flue-cured tobacco.Experimental results show that: compared with CK,bio-organic fertilizer significantly increased the number of bacteria,actinomyce and fungi in the rhizosphere soil,and improved the activities of uraese,polyphenol oxidase and hydrogen peroxidase.Among those different bio-organic fertilizers,bio-organic fertilizer A affected the number of bacteria in the entire tobacco growth process more significantly,and significantly increased the number of bacteria,fungi and actinomycetes in the vigorous period.Moreover,it had much greater impact on the uraese activity in the mid-term of flue-cured tobacco.Bio-organic fertilizer D significantly increased the number of actinomycetes in the vigorous period.From 35 days to 50 days after transplanting,bio-organic fertilizer A,bio-organic fertilizer D increased polyphenol oxidase activity,promoted more organic matter decompose and absorbed by flue-cured tobacco.Bio-organic fertilizer C significantly increased the activity of hydrogen peroxidase in the total growth process of tobacco.Bio-organic fertilizer B only had a greater impact on the actinomycetes,urease,polyphenol oxidase in tobacco early growth stage.Either on micro-organisms or on enzyme activities,T1 had the most obvious effection.