Effect of Shengyangshiyiweiwan on Cell Apoptosis and Cell Cycle in Gastric Cancer

To studay the effect of Inner Mongolia Drug Shengyangshiweiwan on cell apoptosis and cell cycle in humand gastric cancer MGC—803 cell,MTT assay was used to examine the proliferation inhibitory effect of shengyangshiyiweiwan on MGC—803 cell,and calculate the inhibitory rate of growth.Morphologic changes of apoptosis were observed by reverse discrepancy and offluorescent light microscope;flow cytometry was used to analysis cell cycle.MTT assay showed that shengyang has proliferation inhibiting effect on MGC—803 cell in dose and time dependent manners.The cells treated with shengyang(2.5 mg/mL) for 48 hours showed characters of apoptosis under light microscope.An 'sub-G1 peak was detected by flow cytometry,the result analysis of cell cycle indicated that the number of cells of S phase increased,that of G0/ G1 and G2/M phase decreased after treatment on MGC—803,it was arrested S phase.It is conclused that shengyang has proliferation inhibiting effect and may induce apoptosis in gastric cancer MGC—803 cell in a certain dose range,which may be one of the antitumor mechansim.