Comparative Study of Different Extraction Techniques for Extraction of Magnolol and Honokiol from Branches of Megnolia Hypoleuca

Heat reflux,ultrasonic-assisted extraction,microwave-assisted extraction and supercritical carbon dioxide extraction were compared for extraction of magnolol and honokiol from branches of Megnolia Hypoleuca.Reverse-phase HPLC was used for quantitative analysis of magnolol and honokiol.Results showed that among four extraction techniques,the least time was consumed for the microwave-assisted extraction of magnolol and honokiol,and the single microwave-assisted extraction for 40 min at 200 W(1-2.8 PV) with 65% ethanol as extraction solvent using a material/liquid ratio of 1:40(mg/mL) gave the highest yields of magnolol and honokiol,which were valued at 7.893 mg/g and 1.938 mg/g,respectively.