Effect of procyanidin on expression of NF-κB and cerebral infarction volume following cerebral ischemic reperfusion injury in rats

Objective:To study effect of procyanidin on expression of Nuclear Transcription Factor p65(NF-κB p65) and cerebral infarction volume following cerebral ischemic reperfusion injury in rats.Methods:In this experiment,rats were randomly divided into 4 groups,which were sham operated group,I/R group,low procyanidin treated group and high procyanidin treated group.The focal middle cerebral artery occlusion(MCAO) model was made by suture-occluded method.After 90min MCAO following 24h of reperfusion,expression of NF-κB p65 was investigated with imunohistochemistry.TTC staining was also investigated.Results: ①Compared with sham operated group,the translocation of NF-κBp65 from cytoplasm to nucleus increased significantly at 24h of reperfusion in the frontal and parietal cortex(P0.05).Compared with I/R group,low and high procyanidin treated group dose-dependently reduced expression of NF-κBp65.(all P0.05).②Compared with that of I/R group,cerebral infarction volume of low and high dose procyanidin treated group was decreased dose-dependently(all P0.05).Conclusion:Procyanidin may reduced cerebral ischemia-reperfusion injure by reduced expression of NF-κBp65.