2005•Di-Si Junyi Daxue xuebaoRequires access
Expression of matrix metalloproteinase-2 and-9 in pulmonary fibroblasts of silicotic fibrosis rat model
Hai Wang
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Abstract
AIM: To study the expression of MMP-2 and MMP-9 in fibroblasts of rat silicotic fibrosis model and its significance.METHODS: Fifty pathogen-free Wistar rats were randomly divided into experimental group and control group.Experimental group was instillated with SiO_2,while control group with normal saline.Pulmonary interstitial fibroblasts were isolated on day 1,3,7,14 and 28 after SiO_2 exposure.The protein and mRNA expression of MMP-2 was evaluated by immunocytochemistry and RT-PCR.RESULTS: ①The MMP-2 protein expression in fibroblasts increased on day 1 after SiO_2 exposure and reached 3.887 times(t=23.667,P0.001) that of control group on day 7.The expression of MMP-9 protein also increased on day 1 after SiO_2 exposure and reached 3.103 times that of control group(t=11.399,P0.001)on day 7.② The mRNA expression of MMP-2 and MMP-9 was enhanced in fibroblasts at 24 h after SiO_2 instillation.CONCLUSION: The expression of MMP-2 and MMP-9 in fibroblasts is up-regulated in silicotic fibrosis model.