2011•PubMedRequires access

[Effect of tissue factor in extravascular migration of fibrosarcoma cells].

Chunshan Han, Xin Hua, Zhen-Guo Han, Zhongli Gao

Abstract

OBJECTIVE: To evaluate the effect of tissue factor (TF) in extravascular migration of fibrosarcoma cells and hematogenous metastasis. METHODS: The expression of tissue factor in fibrosarcoma HT1080 cells was analyzed by flow cytometry. The extravascular migration of fibrosarcoma cells was observed in a constructed monolayer vascular endothelial cells and extra-cellular matrix model. RESULT: Tissue factor was highly expressed in HT1080 cells. HT1080 migrated and passed through the monolayer vascular endothelial cells to the collagen gel in a time-dependent manner. Anti-TF antibody inhibited extravascular migration of fibrosarcoma cells and the inhibition was concentration-dependent (P<0.05). CONCLUSION: Tissue factor may enhance hematogenous metastasis through extravascular migration of fibrosarcoma cells.

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Key concepts: HT1080, Fibrosarcoma, Tissue factor, Flow cytometry, Pathology, Metastasis, Chemistry, Heparin