Research of Real-Time Fluorescence PCR Detection on Dengue Virus

Objective: Establishing the fluorescence quantitative PCR detection technology of dengue virus.Method: Using highly conserved sequence of 3 'UTR in 1-4 types to design primers and probe for dengue virus,establishing the real-time fluorescent PCR method to detect and quantify the dengue virus.And using the method to test 10 clinical serum samples that ELISA assay has tested positive,which were collected from the fever patients of 1 to 2 days.Results: Using real-time fluorescence PCR to detect four types of dengue virus,all were positive.The probes and primers are universal ones for dengue virus detection.Using this method to detect 10 clinical sera,6 were positive and the positive rate was 60%.Conclusion: Real-time PCR method is a fast,specific,sensitive detection method for dengue virus.It's applicable for the early clinical diagnosis of dengue fever and there are very good social and economic benefits.