Determination of 4 carbamate pesticides residues in cucumber by high performance liquid chromatography-tandem mass spectrometry

A high performance liquid chromatography-tandem mass spectrophy(HPLC-MS/MS) was developed for determination of four carbamate pesticides of aldicarb sulfone,propoxur,isoprocarb,fenobucarb residues in cucumber.The samples were extracted by acetonitrile,and purified by C18 solid-phase extraction column.Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution.The analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization(ESI+) and multiple reaction monitoring(MRM) mode.A matrix matched external standard was used for quantitative analysis.The calibration curves were in good linearity between the peak area and the concentration of 2~200 ng/mL for four carbamate pesticides with correlation coefficients not less than 0.9976.The spiked recoveries ranged from 90.2% to 105.1% with relative standard deviations of 1.9%~6.1%.The proposed method was simple,rapid,sensetive and accutate,and it could fit for the requirements of residue analysis.