Application of fluorescence in situ hybridization technique detecting abnormalities of chromosome 5,7 and 8 in myelodysplastic syndrome

Objective To identify the abnormal karyotypes by fluorescence in situ hybridization(FISH) and explore prognostic implications in patients with myelodysplastic syndrome(MDS).Methods Conventional chromosome analysis(CCA) and FISH were used to detect the frequently chromosome abnormalities(-5/5q-,-7/7q-,+8) in 30 MDS cases,and to analyze the correction of karyotype abnormalities and progress,prognosis of disease by sorftware SPSS11.5.Results The karyotype abnormalities were detected by CCA and FISH as follow:-5/5q-(6.7%) vs(16.7%);-7/7q-(6.7%) vs(16.7%);+8(16.7%) vs(30.0%).Complex karyotypes were associated with the poor prognosis.Conclusion FISH was more sensitive than conventional cytogenetics for detecting mini-clonal abnormality.Multiprobes used in cytogenetic detections may predict the patients′ prognosis more accurately.