Determination of Nucleoside and Base in Cordyceps Health Products

Objective: By using a quantitative valve to introduce sample into the capillary, the injection volume of quantitative capillary electrophoresis(qCE) is fixed, so quantitative results are more reliable and reproducible. This article explored the feasibility of determination of cordycepin, adenine, adenosine, uridine and uracil in cordyceps health product by quantitative capillary electrophoresis(qCE). Methods: The content of cordycepin, adenine, adenosine, uridine and uracil in cordyceps health product was determined at 40 mM borax buffer( pH 9.5) and separation voltage-15 kV. Results: The five kinds of nucleoside and base achieved a good separation, and RSD was lower than 1.4% in terms of peak area. The concentration of these substances were determined in real samples. Conclusion: Cordycepin, adenine, adenosine, uridine and uracil has been quantitative determination by qCE for the first time, the types and quantity of nucleoside and base from different products are different. This method is rapid, accurate, and important for the quality control of Cordyceps health products.