The changes and significance of the energy mechanism in ovary during liver ischemia and reperfusion injury in rats

Objective: The changes of myeloperoxidase(MPO) histology and morphology in liver ischemia-reperfusion (I/R) injury were observed, the relationship with the energy metabolism in ovarian were investigated and its mechanism were studied following the process of liver I/R in rats. Methods: A model of liver I/R injury was established by imitation. Forty eight female Wistar rats were randomly divided into 6 groups as following (n=8): the control group (CTL), the group of sham operateration, simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 2 hour (I/R 2 h), 4 hours (I/R4 h) and 6 hours (I/R6 h) group. The changes of MPO and MDA in ovarian and the changes lactic dehydrogenase (LDH), lactic acid(LD) were measured. Radioimmunoassay (RIA) was used to determine the serumal levels of estradiol and progesterone. Results: During the process of liver I/R. The level of MPO had obvious statistical significant difference in I/R, I/R 2 h group compared with I, I/R 6 h (P0.05). The level of MDA, the serumal level of estradiol and progesterone had obviously statistical significant in I/R, I/R 2 h group compared with I/R 4 h, I/R 6 h (P0.05) group. There was a positive correlation between MPO and MDA (r=0.814, P0.05). The activity of LDH, the serumal level of progesterone in I/R and I/R2 h group were higher than that in CTL and I group (P0.05). The amount of LD was obviously statistical significant between I/R group and I/R2 h, I/R4 h, I/R6 h group (P0.05 ). Conclusion: Estradiol and progesterone may play a protective role in ovarian in rats during the period of original liver ischemia-reperfusion; The change of MPO in ovarian may be involved in the pathogenesis of the ovarian injury and the disorder of energy metablism in ovarian was obvious after reperfusion induced by liver ischemia.