Construction and biological identification of eukaryotic expression vector of PcDNA3.1-rhGM-CSF

Objective To construct eukaryotic expression vector of PcDNA3.1-hGM-CSF and investigate the effect of recombinant vector on suppressing K562 cell growth.Methods The eukaryotic expression vector PcDNA3.1-hGM-CSF was constructed with PCR,T-A clone and directional cloning techniques,and transfected into K562.RT-PCR was used to affirm the expressing of the PcDNA3.1-rhGM-CSF in K562.The cell proliferation assay and immunohistochemistry were used to observed the effect of PcDNA3.1-rhGM-CSF on growth and differentiation of K562 cell.Results The recombinant eukaryotic expression vector PcDNA3.1-GM-CSF was constructed successfully.Recombinant vector PcDNA3.1-GM-CSF was expressed in transfected K562 cells.Transfected K562 cell could differentiate into monocyte and macrophage cell.Conclusion The recombinant eukaryotic expression vector PcDNA3.1-GM-CSF was successfully constructed..