Floral organ tissue culture of oriental lily "Marco polo"
Bin Li
Abstract
Bin Li
Abstract
The clone propagation of oriental lily Marco polo was developed through culture of petal,filament,receptacle,ovary in vitro.The results showed that the best inducement mediums for shoots of receptacle,shoots of petal,shoots of filament and shoots of ovary were MS+6BA 0.5~3.0 mg/L+NAA 0.1~0.2 mg/L,and MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,MS+6-BA 2.0 mg/ L+NAA 0.2 mg/L and MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,respectively.Proliferation medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.Inducement of rooting and bulbing was better in medium of 1/ 2 MS +sugar 50~80 g/L+NAA 0.4~0.8 mg/L.
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The clone propagation of oriental lily Marco polo was developed through culture of petal,filament,receptacle,ovary in vitro.The results showed that the best inducement mediums for shoots of receptacle,shoots of petal,shoots of filament and shoots of ovary were MS+6BA 0.5~3.0 mg/L+NAA 0.1~0.2 mg/L,and MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,MS+6-BA 2.0 mg/ L+NAA 0.2 mg/L and MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,respectively.Proliferation medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.Inducement of rooting and bulbing was better in medium of 1/ 2 MS +sugar 50~80 g/L+NAA 0.4~0.8 mg/L.
Key concepts: Petal, Shoot, Receptacle, Botany, Biology, Tissue culture, Ovary, Murashige and Skoog medium