Depressive effect and expression of Id4 mRNA of arsenic trioxide on Raji lymphoma cells

OBJECTIVE:To study the effect of arsenic trioxide on the treatment of malignancy lymphoma and Id4. METHODS:Human Burkitt's Raji lymphoma cells were cultivated in vitro. Two groups were set up as the control group and the experiment group that was treated with various concentrations and time of arsenic trioxide. The growth of the cells was tested by MTT colorimetry,and the apoptosis and cycle distribution of cells affected by arsenic trioxide were detected by flow cytometry (FCM). The expression of Id4 mRNA in Raji cells treated with arsenic trioxide was detected by RT-PCR. RESULTS:MTT showed that in the experimental group,the cell inhibitive rates of Raji after treated with various concentrations of arsenic trioxide for 24,48,72 hours were 12.15%-92.17%(P0.05). FCM showed that apoptosis peak emerged significantly after Raji cells treated with arsenic trioxide for 48 hours in the experimental group,but there was no or only a low apoptosis peak in the control group. FCM showed that after Raji cells treated with various concentrations of arsenic trioxide for 48 hours in the experiment group,the cell percentage in G0/G1 phase,S phase,G2/M phase of the cell cycle distribution existed a significant difference compared with the control group. Arsenic trioxide could arrest cell cycle,the stoppage increased gradually with arsenic trioxide concentrations increasing,which was dose-deendent. As compared with the β-actin,the expression of Id4 mRNA in Raji cells after treated with arsenic trioxide increased gradually with arsenic trioxide concentrations increasing,which was also dose-dependent. CONCLUSIONS:The Raji cells are depressed effectively by arsenic trioxide in vitro. Arsenic trioxide can induce the apoptosis in Raji cells,with the increase of Raji cells in G0/G1 phase,and decrease of Raji cells in S and G2/M phase. Arsenic trioxide can recover the expression of Id4 mRNA with a dose dependent manner.