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Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography

Dong Bo-ni

Abstract

High-speed countercurrent chromatography was used to separate chlorogenic acid from 70% ethanol extract of dry Lonicera japonica Thunb.with three different solvent systems.It was found experimentally that the optimal solvent system is composed of butanol,acetic acid and wate,with the ratio of 4:1:5(V/V).The purity of obtained chlorogenic acid is determined as 98.1% by high performance liquid chromatograph(HPLC),and its yield is 95%.

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Key concepts: Chlorogenic acid, Chromatography, Countercurrent chromatography, Chemistry, Acetic acid, High-performance liquid chromatography, Countercurrent exchange, Yield (engineering)