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2008•Journal of Xinjiang Agricultural UniversityRequires access

Identification and Cluster Analysis of RAPD-cultivar of Cicerarietinum L.

Shi Qing-hua

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Abstract

Six chickpea total DNAs were extracted by CTAB method and used for identification and cluster analysis was made based on RAPD method.Out of 113 random primers,7 were selected to generate stable and polymorphic DNA fragments.Total 29 RAPD fragments were amplified,16 of which were polymorphic.Each primer could generate 3 to 7 DNA fragments.The percentage of polymorphic loci was 55.17%.The results analyzed by DPS version software showed that Jaccard similarity coefficients ranged from 0.4286 to 0.8462,whose mean was 0.5416,indicating that the genetic variations existed among the six chickpea.A dendrogram was established,based on Hier archical cluster analysis could divide six chickpea cuhivars into 2 groups,among which there were some genetic relations.

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What this paper is about

Six chickpea total DNAs were extracted by CTAB method and used for identification and cluster analysis was made based on RAPD method.Out of 113 random primers,7 were selected to generate stable and polymorphic DNA fragments.Total 29 RAPD fragments were amplified,16 of which were polymorphic.Each primer could generate 3 to 7 DNA fragments.The percentage of polymorphic loci was 55.17%.The results analyzed by DPS version software showed that Jaccard similarity coefficients ranged from 0.4286 to 0.8462,whose mean was 0.5416,indicating that the genetic variations existed among the six chickpea.A dendrogram was established,based on Hier archical cluster analysis could divide six chickpea cuhivars into 2 groups,among which there were some genetic relations.

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Available abstract

Six chickpea total DNAs were extracted by CTAB method and used for identification and cluster analysis was made based on RAPD method.Out of 113 random primers,7 were selected to generate stable and polymorphic DNA fragments.Total 29 RAPD fragments were amplified,16 of which were polymorphic.Each primer could generate 3 to 7 DNA fragments.The percentage of polymorphic loci was 55.17%.The results analyzed by DPS version software showed that Jaccard similarity coefficients ranged from 0.4286 to 0.8462,whose mean was 0.5416,indicating that the genetic variations existed among the six chickpea.A dendrogram was established,based on Hier archical cluster analysis could divide six chickpea cuhivars into 2 groups,among which there were some genetic relations.

Key concepts: RAPD, Jaccard index, Dendrogram, Primer (cosmetics), Biology, Similarity (geometry), Genetic similarity, Genetics

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Identification and Cluster Analysis of RAPD-cultivar of Cicerarietinum L. — Research Paper | ScholarLens