Prokaryotic Expression of the Recombinant σC Protein of Avian Reovirus and the Polyclonal Antibodies Preparation

σC gene of avian reovirus was amplified by RT-PCR and cloned into prokaryotic expression vector pET-30a based on restriction enzyme analysis. The recombinant expression vector pET-30a-σC was transformed into the competent BL21 (DE3). The fusion protein was expressed after induced by IPTG and presented mainly in inclusion body. Western blot analysis showed that the fusion protein (42 ku)was expressed correctly. The specific antiserum against σC was produced in rabbit immunized three times by the purified fusion protein σC. The antibody titer was 1∶105 in ELISA assay,and the antibody had specific reaction with the protein σC expressed by recombinant Baculovirus. The production of σC protein and antiserum will be helpful for function research of σC protein.