Chemical constituents in ethyl acetate extracting fraction from Salacia amplifolia

s:Objective To study the chemical constituents in the ethyl acetate extracting fraction from Salacia amplifolia.Methods The chemical constituents in the ethyl acetate fraction from S.amplifolia were isolated and purified by chromatography on silica gel,Sephadex LH-20,and ODS C18 reverse phase silica gel columns,and their chemical structures were identified by the physicochemical and spectroscopic data.Results Seven compounds were isolated and identified as 15-hydroxy-24-nor-friedel-5-ene-1,3-dione(1),pyracrenic acid(2),2,3,11-trihydroxy-urs-12-ene(3),methyl-3,21-dihydroxy-olean-12-en-28-oate(4),magellanin(5),daucosterol(6),and D-glycero-D-galacto-heptitol(7).Conclusion Compounds 3—5 and 7 are isolated from the plants in Hippocrateaceae family for the first time and compounds 1 and 2 are isolated from this plant for the first time.