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RNAi Technology and Its Application

Zhendong Chen

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Abstract

RNA interference(RNAi) refers to the gene silencing phenomenon induced by double-stranded RNAs.Its realization is mainly through nuclease incising dsRNA into 21-23 nucleotide,which is a small interfering RNA(siRNA),and siRNA mediates to recognize RNAi and target to homology mRNA.At present,RNAi technology was widely used in the research of gene function and gene therapy and other fields.RNAi technology was so hopeful as to become a powerful tool for gene function analysis in the post-genome era.

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What this paper is about

RNA interference(RNAi) refers to the gene silencing phenomenon induced by double-stranded RNAs.Its realization is mainly through nuclease incising dsRNA into 21-23 nucleotide,which is a small interfering RNA(siRNA),and siRNA mediates to recognize RNAi and target to homology mRNA.At present,RNAi technology was widely used in the research of gene function and gene therapy and other fields.RNAi technology was so hopeful as to become a powerful tool for gene function analysis in the post-genome era.

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Available abstract

RNA interference(RNAi) refers to the gene silencing phenomenon induced by double-stranded RNAs.Its realization is mainly through nuclease incising dsRNA into 21-23 nucleotide,which is a small interfering RNA(siRNA),and siRNA mediates to recognize RNAi and target to homology mRNA.At present,RNAi technology was widely used in the research of gene function and gene therapy and other fields.RNAi technology was so hopeful as to become a powerful tool for gene function analysis in the post-genome era.

Key concepts: RNA interference, RNA silencing, Gene silencing, Gene, Small interfering RNA, Biology, Trans-acting siRNA, DNA-directed RNA interference

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