Study on determination of melamine in food by high-performance liquid chromatography

Objective:To establish an analytical method for the determination of melamine in food by high-performance liquid chromatography.Methods:The melamine was separated on Luna NH2 column(250 mm×4.6 mm i.d.,5 μm)by using 90% acetonitrile as an eluent at a flow rate of 1.0 ml/min.Detection was performed on an ultraviolet detector.The wavelength was 240 nm.Quantification was calculated by an external standard method.Results:The linearity in the tested concentration range was good and the correlation coefficient was more than 0.9990.The limit of quantification was 0.1 mg/kg for the target compounds.The extraction recoveries were 82.5%～99.4% and the RSD less than 5.0%.Conclusion:This method is simple,sensitive and accurate for the determination of the melamine in food and it can be satisfactorily applied to the routine analysis.