2009•Zhonghua laonian xin-nao-xueguanbing zazhiRequires access
Mechanism of protective effect of dl-butylphthalide on rat brain after chronic cerebral ischemia
Shuxia Wang
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Abstract
Objective To observe the effect of dl-butylphthalide on learning,memory,expression of p-CREB and BDNF in hippocampus of aged rats after chronic cerebral ischemia.Methods Eighty healthy Wistar rats were selected and divided randomly in to 4 groups of 20 rats each:group A: control group(sham operation);group B:ischemia group;group C:ischemia and low dose treat- ment group;group D:ischemia and high dose treatment group.Chronic cerebral ischemia rat model was established by permanent occlusion and snip of bilateral common carotid arteries(2VO).Cog- nitive ability of all rats was evaluated by Morris water maze test before 2VO,two months and three months after 2VO.Three months after the operation,immunohistocbemical technique was used to observe the effect of dl-butylphthalide on p-CREB and BDNF in hippocampus.Results Compared with the control group,the expression of p-CREB and BDNF in the CA1,CA3 and DG of hippocampus were markedly decreased in the ischemia group.The expression of p-CREB and BDNF in hippocampus of rats in groups C and D were markedly increased as compared with the ischemia group.Compared with group C,the expression of p-CREB and BDNF was markedly in- creased in group D.Conclusion Dl-butylphthalide can improve cerebral ischemia and increase the expression of p-CREB and BDNF.