Effects of siRNA-Smad3 on growth of C2C12 cells induced by TGF-β1

【Objective】To investigate the effects of smad3 on the growth of C2C12 cells induced by TGF-β1. 【Methods】C2C12 cells were transfected with siRNA-Smad3 in vitro, and the cell proliferations were examined with 3H-Thymidine methods. 【Results】After the C2C12 cells interfered with different concentrations of TGF-β1 (0, 1, 5 and 10 ng/mL) for 24 h, the [3H] values (cpm/min) were (630±17), (907±19), (1493±25), (1808±24) respectively, F= 3080.499, P =0.000. While the C2C12 cells were transfected with 200 pmol/L of siRNA-Smad3 for 24 h and then treated with 5 ng/mL TGF-β1 for 24 h,the [3H] values (cpm/min) were (464±30) (blank control group), (1062±50) (internal control group), (428±38) (experimental group), (F =412.186, P =0.000). 【Conclusion】The TGF-β1 could promote C2C12 cells proliferation by Smad3 and showed dose dependent manner. The siRNA-Smad3 could block the signal transduction and decreased C2C12 cells proliferation effectively.