Effects of Mixed Skin Grafting with Fresh Human Amniotic Particles and Autologous Microskin on Wound Healing in Rats

Objective To observe the role of mixed skin grafting with fresh human amniotic granules and allogenous microskin on promoting wound repair.Methods Full thickness skin(4 cm×4 cm) were cut from the back of 24 SD rats.The rats were divided into 3 groups with 8 rats in each group.Allogenous microskin group was transplanted with 0.1 g allogenous microskin.Treatment group 1 and 2 were grafted with mixed autologous microskin and fresh human amniotic granules with the ratio 1∶2(0.1 g∶0.2 g) and 1∶4(0.1 g∶0.4 g).Twenty-one and 28 days after skin grafting,the wound healing status in general was observed and histological examination was performed.The rates of wound healing and contraction were measured.Results ① 21 days after surgery,the healing rates of treatment group 1 and 2 were higher than that in allogenous microskin group.In all the groups,the wound contraction was not obvious,the wounds were covered by granulation tissue,a little transudation was observed,epidermal cells were proliferous,and the blood capillaries were rich in dermis.But in allogenous microskin group,fibroblasts and blood capillaries were greater in dermis than the two treatment groups,the arrangement of collagen fibers was inordinate,and a few inflammatory cells were infiltrated.Wound healing rates: treatment group 2 treatment group 1(P0.05),treatment group 1 and 2 allogenous microskin group(P0.05).Wound shrinking rates:treatment group 2 treatment group 1(P0.05),treatment group 1 and 2 allogenous microskin group(P0.05).②28 days after surgery,the wounds were well-healed in two treatment groups.The healiing rate of allogenous microskin group were higher than that 21 days after surgery,the exposed wounds were covered by granulation tissue,and abundant transudation was observed.The shrink degree was less in two treatment groups than that in the allogenous microskin group.In two treatment groups,epidermal hyperplasia was greater than that in the allogenous microskin group,the collagen fibers lined up in order in dermis,and the joints of epiderma and dermis had obvious spikes.In allogenous microskin group,the blood capillaries were rich in dermis,the inflammatory cells were infiltrated,and the proliferation of fibroblasts was obvious.Wound healing rates: treatment group 2 treatment group 1(P0.05),treatment group 1 and 2 allogenous microskin group(P0.05).Wound shrinking rates:treatment group 2reatment group 1(P0.05),treatment group 1 and 2 allogenous microskin group(P0.05).Conclusion Mixed skin grafting with fresh human amniotic amniotic particles and autologous microskin can promote wound healing.