Effects of exogenous uteroglobin gene on cell apoptosis and proliferation of human cervical cancer cell line HeLa

Objective To investigate the effects of exogenous wild uteroglobin(UG)gene stable transfection on cell apoptosis and proliferation in human cervical cancer cell line HeLa.Methods UG recombinant eukaryotic expression vector pcDNA3.1-UG(+)was constructed,and then stably transfected into cultured human cervical cancer HeLa cells by liposome mediation(pcDNA3.1-UG(+)/HeLa group).Simultaneously,empty vector pcDNA3.1 plasmid was transfected(pcDNA3.1/HeLa group).Non-transfected HeLa cells were considered as controls(HeLa group).RT-PCR and Western blotting assay were applied to detecting the expression of targeted gene and protein,and MTT reduction assay and flow cytometry were applied to measuring cell proliferation and apoptosis.Results UG mRNA and protein were high expressed in the stably transfected cells(pcDNA3.1-UG(+)/HeLa group),significantly higher than those of control group(P0.05).The proliferation rate of cells transfected with pcDNA3.1-UG(+)plasmid was obviously lower as compared with that of cells transfected with empty vector.The apoptotic rate of pcDNA3.1-UG(+)/HeLa cells was markedly higher than that of non-transfected HeLa cells.By contrast,there were no significant changes of the cell growth velocity and apoptotic rate in pcDNA3.1/HeLa group.Conclusion The transfection of UG gene might suppress the growth and induces the apoptosis of human cervical cancer cell line HeLa cells.