Proliferation and Identification of the Dendritic Cells from the Peripheral Blood of Patients with Cervical Cancer

Objective To culture and proliferate dendritic cells from the peripheral blood of patients with cervical cancer, to observe the morphologic, phenotypic characteristics of these cells and its potentiality to stimulate the proliferation of allo-T lymphocytes. Methods Monocytes were isolated from the peripheral blood of patients and cultured with the cytokines(GM-CSF,IL-4,TNF-а).The morphologic characteristics of those cells were observed with optics and electron microscopes and the phenotypic figures were analyzed with FACS. The proliferating activity of T lymphocytes was determined by MTT in vitro. Results Large amount of mature DCs could be obtained from the peripheral blood of patients with cervical cancer. DCs expressed high level of HLA-DR、CD1a、CD80,and were potent to stimulate the proliferation of allo-T cells. Conclusion The mature dendritic cells could be obtained by culturing the peripheral blood of patients with cervical cancer, which set up the further foundation for clinical biotherapy of cervical cancer.