Multifunctional Column Clean-up and Determination of Aflatoxin M_1 in Milk by Liquid Chromatography-tandem Mass Spectrometry

A simple and rapid determination method of aflatoxin M1 in milk by liquid chromatography-tandem mass spectrometry has be established.The samples were extracted by acetonitrile/water(V/V,86/14),226 multi-function columns were used as purification technology.After centrifugation,drying,constant-volume and filtration,the samples were detected by liquid chromatography-tandem mass spectrometry with C18 column(Waters,Xtera,150 mm×2.1 mm×5 mm).0.1% Formic acid and acetonitrile/methanol(V/V,50/50) were selected as mobile phases.The samples were detected by external standard method.The results of methodology verification were as follows: LOD=0.15 ng/mL,LOQ=0.5 ng/mL,the linear correlation R=0.996 1.The recovery experiments were performed by blank sample spiked at the two levels,the recoveries were 71.5% and 83.5%,with the relative standard deviations(RSD,n=6) were 2.7% and 8.4%.The advantages of this method included simple pre-treatment,high speed and sensitivity.It was suitable for aflatoxin M1 detection in milk.