Expression and significance of apoptosis related protein in spinal cord after hind limbs ischemia-reperfusion

Objective To evaluate the expression and significance of apoptosis related protein,including Bcl-2 and Bax after hind limbs ischemia-reperfusion(LIR) in rats spinal cord.Methods The limbs ischemiareperfusion model was established in rats.The histo pathological changes of spinal cord were determined by Nissl staining,apoptotic cells were detected with TUNEL,the expression of apoptosis associated protein,such as Bcl-2 and Bax were determined with immunohistochemistry.Results The expression of Bcl-2 and Bax following LIR significantly increased,peaked at 12h and gradually decreased.The Bcl-2 /Bax ratio kept minimal at 24h,while apoptotic cells were most,Bcl-2/Bax ratio and apoptotic cells was negatine correlation.Conclusion Hind limbs ischemia-reperfusion(LIR) lead to spinal cord injury and the apoptosis of neural cells.The expression of apoptosis-associated factors,including Bcl-2 and Bax may play a vital role in regulating the neural apoptosis following LIR in rats.