Effect of human oviductal fluid and oviductal epithelial cell cultural medium on human sperm acrosome reactions

Aim: This study was to investigate the effect of human oviductal fluid (OF) and oviductal epithelial cell cultural medium (OECM) on human sperm acrosome reactions. Methods: With Ham's F10 as control group, OF and OECM as experimental groups, Co-cultural experiments with sperm of fertile group (FG, n =20) and infertile group (IG, n =20) were carried out. Acrosome reaction was evaluated by triple-stain method. Results: OF and OECM enhanced the acrosome reaction rate ( P 0 05) for both FG and IG, compared with the control group within 3 h treatment. After treated for 6 h, the acrosome reaction rates of OECM for FG and IG had higher values than those of OF. Besides, it was observed that the death rate of sperm undergoing acrosome reaction sperm of IG was higher than that of FG. Conclusion: OF and OECM significantly improved human sperm acrosome reaction which was easily induced to sperm of infertile men. But such sperm would easily die after acrosome reaction. [