Development of real-time quantitative PCR assay of porcine circovirus type 2

A segment of 417 base pair(bp) for porcine circovirus type 2(PCV2) gene was amplified by PCR assay from the tissues of a clinical diseased pig,and was cloned into the pGEM-T easy vector.Then,one recombinant plasmid with the 417 bp segment was constructed.The standard curve and the corresponding linear regression equation of PCV2 DNA level were obtained by real-time fluorescent quantitative PCR with the recombinant plasmid.The results showed that this method was easily reproducible and high specific while used to detect samples,its sensitivity was proved to be 102 copies/L.The present study indicated that the real-time PCR assay of PCV2 DNA was of high specificity,sensitivity and reproducibility,and provided a method to quantify PCV2.