2008•Biotechnology(Faisalabad)Requires access

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus LJL12 Strain

Zhou En-min

Abstract

Objective:We determinate the sequences of NS1 Gene of Porcine Parvovirus LJL12 strain and analyze homologies of nucleotide sequences and amino acid sequences.Methods: According to the NS1 sequence of PPV Chinese strain in the GenBank,a pair of specific primers was designed to amplify the NS1gene of the LJL12 strain.Molecular biological soft wares were used to analyze homologies.Results: Integrated sequence of NS1gene has 1989 base pair,encodes 662 amino acid,exhibited 98.6% to 100% nucleotide sequence identity and 98.5% to 100% amino acid identity with other Chinese strains.The highest is the Nanjing strain among these.Conclusion: NS1 gene is highly conservative.It can be used in diagnosis.Molecular cloning of LJL12 NS1 gene makes it possible to disclose the function of NS1 protein.

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Key concepts: GenBank, Cloning (programming), Gene, Genetics, Biology, Porcine parvovirus, Nucleic acid sequence, Strain (injury)