Antitumor Effects of Cocultured Dendritic Cells and Cytokine-induced Killer Cells on Breast Cancer

Objective:This study was aimed to investigate the phenotype,proliferative activity,and cytotoxicity of CIK cells after in vitro co-culturing with DCs.Methods: DCs and CIK cells were prepared from human PBMCs, then CIK cells were cocultured with autologous DCs for 4 days at a stimulator-to-responder ratio of 10:1 to prepare immunologic effector DC-CIK cells.Detected the proliferation activities,phenotypes and in vitro cytotoxicity of DC-CIK cells.Results: Compared with CIK cells and DC-CIK cells got a significant increase of proliferation rate,and resulted in an enhancement of cytotoxicity to SKBR-3 cells in vitro.Conclusion: CIK cells have high lytic activity against SKBR-3 cells,which can be enhanced by co-culture with DCs.DC-CIK cells are highly effective immune cells.