2006•Neural Injury and Functional ReconstructionRequires access
Effect of Olomoucine on Astrocyte Proliferation and Scar Formation after Focal Cerebral Ischemia
Guibin Zhang
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Abstract
Objective: The effect of a cell cycle inhibitor,olomoucine,on astrocytic proliferation was studied by measuring astrocytic proliferation and scar formation after focal cerebral ischemia in rats.Methods: An ischemic rat model was generated by photochemistry method.The expression of glial fibrillary acidic protein(GFAP) was studied by immunofluorescence.Protein levels for GFAP,proliferation cell nuclear antigen(PCNA),cyclin A and cyclin B1 were investigated by Western blot at days 3,7 and 30 after operation. Results: Olomoucine significantly reduced the expression of GFAP.In parallel study,glial scar tissue was observed near infarct regions in control rats at 7 and 30 days,and its density of scar formation was significantly greater at 30 days than at 7 days.Olomoucine significantly inhibited scar formation.Western blot showed increased protein levels for GFAP,PCNA,CyclinA and CyclinB1 in control rats,while the up-regulation of the proteins above was inhibited by olomoucine.Conclusion: The study suggested that olomoucine could significantly inhibit activation,proliferation and scar formation of astrocytes.Regulating cell cycles may provide a new method for treating cerebral ischemia.