2005•Zhongguo shouyi ke-jiRequires access
Development of a triplex PCR assay for detection of porcine circovirus type 2,pseudorabies virus and porcine parvovirus
Fang Li, Xu Hui, Weijie Chen, Lingyan Zhao, Ni Baifeng, Fang Wei-huan
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Abstract
A triplex PCR assay was developed and evaluated for the simultaneous detection of infections with porcine circovirus type 2(PCV2),pseudorabies virus(PRV) and porcine parvovirus(PPV).Specific primers for each of the three viruses were designed and used.The lowest detection limit of the triplex PCR was 4×10~(-4)ng/μL approximately for double-strained DNA template of PRV and 2×10~(-5)ng/μL for single-strained DNA template of PCV2 and PPV,when a composite of the three viruses was tested as a single sample.This assay was also effective for detecting one or two of the three viruses in various combinations in 21 tissue specimens collected from diseased pigs and aborted fetuses.Results suggested that the established triplex PCR assay had potential for clinic application.