2004•Industrial MicrobiologyRequires access

Cloning of alcohol dehydrogenase II gene from Zymomonas mobilis and expression in Escherichia coli

Jian Lü

Abstract

The gene adhB encoding alcohol dehydrogenaseⅡ(ADHⅡ)of Zymomonas mobilis was amplified by PCR from total DNA . The recombinant plasmid,pSE-adhB was constructed by inserting adhB into expression vector pSE380 and then transformed E.coli DH5α. The recombinant strains were induced by IPTG to express ADHⅡ. The activity was determined by aldehyde indicator plates. Expression level of recombinant alcohol dehydrogenaseⅡ(ADHⅡ) reached 5u/mL.By companision, no activity appeared in hosted E.coli DH5α.

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Key concepts: Zymomonas mobilis, Alcohol dehydrogenase, Escherichia coli, Recombinant DNA, Cloning (programming), lac operon, Plasmid, Molecular biology