Effect of parecoxib pretreatment on the expression of GFAP,IL-1β and TNF-α in rat brain against focal cerebral ischemia-reperfusion injury in rats

Objective To investigate the effect and the mechanism of parecoxib pretreatment on brain against focal cerebral ischemia-reperfusion(IR) injury in rats.Methods Sixty-four male SD rats weighing 250-300 g were randomly divided into four groups (n=16 each): shame operation (group S); focal cerebral IR(group IR); parecoxib 5/10 mg/kg(30 minutes before ischemia)+focal cerebral IR(group P5, P10). Middle cerebral artery occlusion (MCAO)models were made by reforming Longa suture method in SD rats. The infarct volume and the infarct volume fraction were detected by TTC staining, the neurologic deficit scores(NDS) in each group was recorded at 2 h of occlusion and 24 h of reperfusion, the pathological changes in CA1 region of hippocampus were detected by HE staining, radioimmunoassay technique was used to determine the content of PGE2,IL-1β,TNF-α in rat brain, the levels of protein GFAP in AS were detected by immunohistochemistry.Results The NDS were higher in group IR,P5,P10 than in group S at 2 h of ischemic and 24 h of reperfusion(P0.05 or P0.01). The NDS were lower in group P10 than that in group IR at 24 h of reperfusion(P0.05). The infarct volume was significantly smaller in group P5,P10 than in group IR(P0.01). The content of PGE2,IL-1β,TNF-α in brain in group P5,P10 were significantly lower than in group IR,and the group P10 was significantly lower than the group P5. The number of GFAP positive cells in CA1 region of hippocampus in group IR is more than group S, P5,P10 were significantly less than in group IR and group P10 significantly less than in group P5(P0.05 or P0.01).Conclusion Pretreatment with parecoxib can protect the brain from focal cerebral IR injury by reduced the content of PGE2, inhibit the overactivation of AS, reduce IL-1β,TNF-α release in rat brain.