The significance of tumor necrosis factor-α and interleukin-6 in experiment of myocardium hypertrophy

Objective To investigate the expression of change and significance about tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) in serum and myocardial of myocardial hypertrophy rats.Methods 20 Wistar rats were randomly divided into two groups: myocardial hypertrophy group(OG) and control group(CG).Each group contained 10 rats.The partial abdominal aorta ligature was used to induce hypertrophy in rats.After surgery,rats were feeded according to the requirements for eight weeks,then put to death.Blood and myocardial specimens were obtained.TNF-α and IL-6 content in serum were detected with radioactive immune method.The expression changes of TNF-α mRNA and IL-6 mRNA in myocardial tissue were detected with RT-PCR method.Results Compared with control group,it was found that in myocardial hypertrophic group the content of TNF-α and IL-6 in serum increased greatly(P 0.05);the express of myocardial of TNF-α mRNA and IL-6 mRNA increased(P 0.05).Conclusion TNF-α and IL-6 play a role in myocardial hypertrophy pathogenesis.