Expression of RUNX3 Gene in Breast Carcinoma Tissues and in Breast Cancer Cell Lines and Its Significance

[Purpose] To investigate the role of RUNX3 gene in breast carcinogenesis by analysing the expression of RUNX3 gene mRNA and protein in breast carcinoma tissues and breast cancer cell lines.[Methods] Expression of mRNA and protein of RUNX3 were detected in five breast cancer cell lines,one normal breast cell line,30 pairs of fresh breast cancer tissues and normal breast tissues by RT-PCR and Western Blot methods.[Results] The expression of RUNX3 mRNA and protein were negative in three(T47D,MCF7,SKBR3) of five breast cancer cell lines.Of the 30 pairs of fresh tissues the expression of RUNX3 mRNA and protein in fresh breast cancer tissues was lower than that in normal breast tissues.The expression of RUNX3 was correlated to clinical stage and lymph node metastasis,but not correlated to age and pathological grade.[Conclusion] RUNX3 gene might involve in carcinogenesis of breast cancer acting as a suppressor gene.