The inhibitory mechanism of down-regulation expression of pp-GalNAc-T14 by oridonin on human renal carcinoma cells in vitro

Objective To investigate the inhibitory mechanism of down-regulation expression of polypeptide N-acetylgalactosaminyl-transferase 14(pp-GalNAc-T14) by oridonin on human renal carcinoma cells in vitro.Methods The expression of pp-GalNAc-T14 mRNA was detected by reverse-transcribed polymerase chain reaction(RT-PCR) in 20 resected renal cancer tissue samples and matched normal paraneoplastic tissues.After treated with different concentrations of oridonin,inhibition of human renal cancer cell line(786-0) growth was examined by MTT assay.Then levels of pp-GalNAc-T14 mRNA before and after oridonin treatment was detected by RT-PCR.Results(1) pp-GalNAc-T14 mRNA was detectable in both renal cell carcinoma tissues and matched normal tissues.Compared with the normal control samples,a 34.91% increase of pp-GalNAc-T14 expression in the renal cell carcinoma tissues was observed(P0.05).(2) At 24 hours,48 hours and 72 hours after oridonin treatment in the 786-0 cells,expression of pp-GalNac-T14 mRNA significantly decreased 15.98%,44.30% and 58.61% respectively,compared with control cells(P0.05).(3)The growth and proliferation of human renal cell carcinoma cell line 786-0 could be inhibited by oridonin and showed time-and dose-dependent.Conclusions Oridonin inhibit growth of renal cell carcinoma cells through down-regulating the expression of pp-GalNAc-T14 and may be used as an anti-tumor medicine in the chemotherapy of renal cell carcinoma.