Correlation of sperm DNA fragmentation index and semen parameters

Objectives:To explore the relationship between the male sperm DNA fragmentation,semen parameters and acrosome activity.Method:Semen samples of 553 male patients were collected from our center.Sperm DNA fragmentation index(DFI) was detected by Sperm Chromatin Dispersion(SCD) methods.According to the results of sperm DFI,all the values were divided into group I(DFI 15%),group II(15% ≤DFI 25%) and group III(DFI≥25%),and the sperm density,count,percentage of progressive motility sperm,morphology and acrosome activity were detected at the same time.The correction of DFI and semen parameters were analyzed.Results:Sperm density and total sperm count had no significant correlation with the DFI.The percentage of progressive motility sperm gradually decreased with the augment of DFI,and there were significant differences between group I and group III.Normal sperm morphology rate also decreased with the augment of DFI,and the difference was not significant.Acrosome activity gradually decreased with the augment of DFI as well,with significant differences among the groups.Conclusion:Negative correlations were obtained between both progressive motility sperm and acrosome activity.As a result,DFI is a better reference index for sperm function.