Optimization of process parameters for sequential extraction of protein and chlorogenic acid from sunflower meal

To take NaCl concentration,extraction temperature and solid-liquid ratio as independent variables and extraction rate of protein as response value and ethanol concentration,extraction time and solid-liquid ratio as independent variables and extraction rate of chlorogenic acid as response value,the paper obtained the prediction model of simulated regression equation by using response surface methodology to optimizing the extraction processing parameters of Protein and chlorogenic acid based on the single-factor test.The results showed that the optimum extraction conditions of protein were: NaCl concentration 1.51mol/L,solid-liquid ratio 1∶12.88(g/mL),extraction temperature 60.27 ℃;the optimum extraction conditions of chlorogenic acid were 56.07%of ethanol concentration,solidliquid ratio 1∶16.03(g/mL),47.06min of extraction time.Under these conditions,the extraction rates of protein and chlorogenic acid were 36.64%and 2.73%respectively.