Study on extraction and antioxidant activity of anthra quinones from root of Actinidia arguta by ultrasonic technology

Extraction technology of anthraquinones from root of Actinidia arguta was studied by using ultrasionic sound extraction equipment and taking extraction ratio of flavonoids as marker. Effects of ethanol concentration,ration of material to liquid,extracted time and extracted temperature on extraction rate of anthraquinones from root of Actinidia arguta by single factor test and response surface methodology. Its scavenging ability to DPPH free radical reduction ability to Fe3+and chelating ability to Fe2+were analyzed by chemical simulated system and compared with those of vitamin C. The optimum extraction conditions as follows:ethanol concentration 80%,ultrasionic sound power 200W,extracted time 15min,extracted temperature 70℃. Under these conditions,the extraction rate was up to 1.40%. The extract obtained had a powerful scavenging activity against DPPH free radicals,reducing ferricions and stronger chelating ability in a concentration-dependent manner.